Deciphering zebrafish spectral signatures: insights from Raman spectroscopy

Authors

  • Isabel Oliveira Abreu CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões. Av. Norton de Matos, s/n, 4450-208 Matosinhos, Portugal; Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal; School of Medicine and Biomedical Sciences, University of Porto, Rua de Jorge Viterbo, 228, 4050-313 Porto, Portugal https://orcid.org/0000-0002-6214-9324
  • Cláudia Teixeira CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões. Av. Norton de Matos, s/n, 4450-208 Matosinhos, Portugal; Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal
  • Rui Vilarinho Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal; IFIMUP – Institute of Physics for Advanced Materials, Nanotechnology and Photonics, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal
  • A. Cristina S. Rocha University of Coimbra, Marine and Environmental Sciences Centre/ARNET - Aquatic Research Network, Department of Life Sciences, Calçada Martim de Freitas, 3000- 456, Coimbra, Portugal
  • Joaquim Agostinho Moreira Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal; IFIMUP – Institute of Physics for Advanced Materials, Nanotechnology and Photonics, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal
  • Luís Oliva-Teles CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões. Av. Norton de Matos, s/n, 4450-208 Matosinhos, Portugal; Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal
  • Laura Guimarães CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões. Av. Norton de Matos, s/n, 4450-208 Matosinhos, Portugal
  • António Paulo Carvalho CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões. Av. Norton de Matos, s/n, 4450-208 Matosinhos, Portugal; Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal

DOI:

https://doi.org/10.48797/sl.2024.147

Keywords:

Selected Oral Communication

Abstract

Background: Raman spectroscopy (RS) is a sensitive technique used for gathering chemical and molecular data, resulting in a biochemical fingerprint of the sample. This method is label-free and provides rapid detection [1]. Furthermore, it has proven invaluable in the forensic, biological and medical contexts [2]. It is also suitable for investigations assessing toxic effects of some chemicals, enabling swift acquisition of extensive organism-specific information. Its application to zebrafish serves various purposes, including physiological [3] and toxicological evaluation [4]. However, no base characterization of this organism has been provided in such studies that could be useful for the planning of experiments aiming at diagnosing and follow-up environmental contamination. Objective: The present study aimed to characterize the developmental Raman profile of different zebrafish organs, by drawing a baseline analysis of embryos and larvae up to 168 hpf. Methods: Different organs or tissues were examined daily from 24 to 168 hours post-fertilization, according to their time window of emergence in the embryo, including the heart, muscle, brain, iris, swim bladder, and melanocytes. Chemometric analysis, employing partial least-squares discriminant analysis (PLS-DA), was employed to characterize the organs and ascertain the contribution of spectral bands to their discrimination. Results: A total of 117 spectral bands were identified, with 24 demonstrating robust, systematic representation. The bands were found in the 223 to 3431 cm-1 spectral range; most of them were related to amino acids and relevant macromolecules, such nucleic acids, proteins and lipids. Only three bands were found to be common to all recorded organs and tissues. PLS-DA generated distinct spectral fingerprints for each organ, illustrating variations over early development. Conclusions: Overall, the work developed provided a clear baseline profile of organs and tissues of zebrafish embryos and larvae, identifying Raman bands of expeditious acquisition and their expected variation over different developmental stages, before and after hatching.

References

1. Pinto, R.; Vilarinho, R.; Carvalho, A.P., Moreira, J.A., Guimarães, L., Oliva-Teles, L. Novel approach to freshwater diatom profiling and identification using Raman spectroscopy and chemometric analysis. Water 2022, 14, 2116.

2. Applications. In Modern Raman Spectroscopy-A Practical Approach, 1st ed.; Smith, E., Dent, G., Eds.; John Wiley & Sons Ltd: West Sussex, England, 2005; pp. 135–179.

3. Akiva, A., Kerschnitzki, M., Pinkas, I., Wagermaier, W., Yanik, K., Fratzl, P., Addadi, L., Weiner, S. Mineral formation in the larval zebrafish tail bone occurs via an acidic disordered calcium phosphate phase. J. Am. Chem. Soc. 2016. 138(43), 14481-14487.

4. Han, Y., Qian, J., Zhang, J., Hu, C., Wang, C. Structure-toxicity relationship of cefoperazone and its impurities to developing zebrafish by transcriptome and Raman analysis. Toxicol. Appl. Pharmacol. 2017. 327, 39-51.

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Published

2024-05-01

How to Cite

Oliveira Abreu, I. ., Teixeira, C., Vilarinho, R., S. Rocha, A. C. ., Moreira, J. A., Oliva-Teles, L., Guimarães, L., & Carvalho, A. P. . (2024). Deciphering zebrafish spectral signatures: insights from Raman spectroscopy. Scientific Letters, 1(Sup 1). https://doi.org/10.48797/sl.2024.147

Issue

Vol. 1 No. Sup 1 (2024)

Section

Oral Communications

License

Copyright (c) 2024 Isabel Oliveira Abreu, Cláudia Teixeira, Rui Vilarinho, A. Cristina S. Rocha, Joaquim Agostinho Moreira, Luís Oliva-Teles, Laura Guimarães, António Paulo Carvalho

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