Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2
DOI:
https://doi.org/10.48797/sl.2023.20Keywords:
SARS-CoV-2, saliva, viral transport mediumAbstract
In December 2019, in Wuhan, an outbreak of a new coronavirus (SARS-CoV-2) began to spread rapidly, resulting in a potentially fatal viral respiratory disease. Since August 23, 2020, more than 679 million cases of COVID-19 and nearly 6.8 million deaths have been confirmed in more than 200 countries. This, in turn, had a severe impact on public health and the world economy. The SARS-CoV-2 pandemic also resulted in a shortage of viral transport medium and in the need to find different diagnostic means, such as saliva. To fill this gap in the market, a new viral transport medium was created and tested with samples collected from the nasopharynx and/or oropharynx using swabs and saliva samples. The specificity, sensitivity, and threshold cycles (Cts) of Real-Time PCR (RT-PCR) testing of the samples were compared, revealing concordant results with approximate sensitivity and specificity. Our study highlighted the need to optimize saliva sample collection and its potential use as a substitute for standard collection.
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Copyright (c) 2023 Fernando Ferreira, Daniela Alves, Telma Oliveira, Silvia Coelho, Inês Oliveira, Paula de Melo Alves
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