Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2


  • Fernando Ferreira University Institute of Health Sciences (IUCS) CESPU, Rua Central da Gandra, 1317, 4585-116, Gandra, Paredes, Portugal; CESPU Diagnóstico, SA company, Rua Central de Gandra, 1317, 4585-116, Gandra, Paredes, Portugal https://orcid.org/0000-0002-2071-0750
  • Daniela Alves CESPU Diagnóstico, SA company, Rua Central de Gandra, 1317, 4585-116, Gandra, Paredes, Portugal
  • Telma Oliveira CESPU Diagnóstico, SA company, Rua Central de Gandra, 1317, 4585-116, Gandra, Paredes, Portugal https://orcid.org/0009-0006-1258-5941
  • Silvia Coelho CESPU Diagnóstico, SA company, Rua Central de Gandra, 1317, 4585-116, Gandra, Paredes, Portugal https://orcid.org/0009-0002-0207-3804
  • Inês Oliveira CESPU Diagnóstico, SA company, Rua Central de Gandra, 1317, 4585-116, Gandra, Paredes, Portugal https://orcid.org/0000-0003-3129-8981
  • Paula de Melo Alves Polytechnic Institute of Health of the North (IPSN) CESPU, Rua Central da Gandra, 1317, 4585-116, Gandra,Paredes, Portugal; CESPU Diagnóstico, SA company, Rua Central de Gandra, 1317, 4585-116, Gandra, Paredes, Portugal https://orcid.org/0000-0003-0549-8031




SARS-CoV-2, saliva, viral transport medium


In December 2019, in Wuhan, an outbreak of a new coronavirus (SARS-CoV-2) began to spread rapidly, resulting in a potentially fatal viral respiratory disease. Since August 23, 2020, more than 679 million cases of COVID-19 and nearly 6.8 million deaths have been confirmed in more than 200 countries. This, in turn, had a severe impact on public health and the world economy. The SARS-CoV-2 pandemic also resulted in a shortage of viral transport medium and in the need to find different diagnostic means, such as saliva. To fill this gap in the market, a new viral transport medium was created and tested with samples collected from the nasopharynx and/or oropharynx using swabs and saliva samples. The specificity, sensitivity, and threshold cycles (Cts) of Real-Time PCR (RT-PCR) testing of the samples were compared, revealing concordant results with approximate sensitivity and specificity. Our study highlighted the need to optimize saliva sample collection and its potential use as a substitute for standard collection.


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How to Cite

Ferreira, F., Alves, D., Oliveira, T., Coelho, S., Oliveira, I., & de Melo Alves, P. . (2023). Evaluation of saliva as an alternative to standard collection for detection of SARS-CoV-2. Scientific Letters, 1(1). https://doi.org/10.48797/sl.2023.20



Research Articles