Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves

Carlos Valencia-Salgado; Selene Jacobo-Arreola; Salvado Said-Fernandez; Adolfo Soto-Dominguez; Alberto Camacho-Morales; Herminia Martinez-Rodriguez; Humberto Rodriguez-Rocha; Paulina Delgado-Gonzalez; Adriana Quiroz-Reyes; Jose Islas; Elsa Garza-Treviño

doi:10.48797/sl.2022.3

Authors

Carlos I. Valencia-Salgado Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0003-3470-4105
Selene Jacobo-Arreola Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0003-4866-0959
Salvado L. Said-Fernandez Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0001-8710-9460
Adolfo Soto-Dominguez Departamento de Histologia, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0002-4144-5474
Alberto Camacho-Morales Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0002-2588-9489
Herminia G. Martinez-Rodriguez Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0001-8801-0999
Humberto Rodriguez-Rocha Departamento de Histologia, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico
Paulina Delgado-Gonzalez Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0002-0492-7968
Adriana G. Quiroz-Reyes Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0002-9405-7483
Jose F. Islas Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico
Elsa N. Garza-Treviño Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 64460, Mexico https://orcid.org/0000-0002-1042-4603

DOI:

https://doi.org/10.48797/sl.2022.3

Keywords:

scaffolds, adipose tissue-derived mesenchymal stem cells, oligodendrocyte-like cells, sciatic nerve

Abstract

We evaluated cell markers of oligodendrocyte-like cells (OLC) and neuron-like cells (NLC) differentiation from human adipose tissue-derived mesenchymal stem cells (hADSCs) cocultured on decellularized sciatic nerves. OLC and NLC were incubated at a 10:1 ratio with decellularized rat nerves. We estimated the percentage of myelin basic protein (MBP)- and neurofilament protein (NFP)-positive cells. OLC and NLC cocultures showed an increase in positive cells for MBP and proteolipid protein (PLP) markers. Oligodendrocytes and neurons cocultured with sciatic nerve scaffolds promoted myelination by OLC within 3 days, which remained stable for at least 21 days. We propose a reproducible experimental strategy to promote sciatic nerve myelination by coculturing hADSCs-derived OLC and NLC in nerve scaffolds.

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