Development and validation of an HPLC-DAD analytical method for psilocybin and psilocin
DOI:
https://doi.org/10.48797/sl.2025.330Keywords:
Selected Oral CommunicationAbstract
Background: "Magic mushrooms", also known as hallucinogenic mushrooms, belong to the genus Psilocybe and contain tryptamines, especially psilocybin and psilocin, with potential medicinal benefits for mental health [1]. Psilocybin is usually found in higher concentrations than psilocin and both are present in dried mushrooms at a total concentration of approximately 0.5 to 1.5%. Psilocybe mushrooms are used for religious, recreational, and therapeutic purposes due to their psychedelic effects [1]. The increasing use of these tryptamines and other psychoactive drugs led to regulatory legislation, specifically Portuguese Decree-Law No. 15/93 of January 22, which aims to control the production, distribution, possession, and use of these substances. High-performance liquid chromatography (HPLC) coupled with UV-Vis, diode array (DAD) [2], or mass spectrometry (MS) [3] are commonly used analytical methods to quantify tryptamines. Objective: Development and validation of an HPLC-DAD analytical method for quantification of psilocybin and psilocin in Psilocybe cubensis extracts. Methods: Mushrooms were pulverised using a porcelain mortar and pestle and extracted with cold methanol by kinetic maceration on a magnetic stirrer plate. An Agilent 1260 Infinity II HPLC-DAD system with a Poroshell 120 EC-C18 3.0 x 150 mm, 2.7 µm column protected with a Poroshell 120 EC-C18 3.0 mm, 2.7 µm guard column was used for analytical determinations. The optimised chromatographic method was established with mobile phase solvents: 10 mM ammonium formate with 0.1% formic acid and acetonitrile; oven temperature: 40 °C; flow rate: 0.8 mL/min; total run time: 12 minutes; injection volume: 1 µL; and UV detection wavelength: 220 nm. Results: To optimise the HPLC-DAD analytical method, various parameters were evaluated: mobile phase gradients and solvents, oven temperature, flow rate, run time, post-run time, injection volume and UV detection wavelength. This analytical procedure was validated following the ICH Q2 guideline. Linearity was tested over a range of 9 concentrations (0.50-200 µg/mL for psilocybin and 0.25-100 µg/mL for psilocin), obtaining R2 values > 0.999. Method precision (%RSD) was £ 10%, with an accuracy (%bias) £ 15% for both compounds. Conclusions: An HPLC-DAD analytical method for the detection and quantification of psilocybin and psilocin was optimised, validated, and effectively applied to Psilocybe cubensis extracts.
References
1. Tylš, F. et al. Psilocybin – Summary of Knowledge and New Perspectives. Eur Neuropsychopharmacol 2014, 24, 342–356, doi: 10.1016/j.euroneuro.2013.12.006.
2. Foster, K. et al. The Effect of Casing and Gypsum on the Yield and Psychoactive Tryptamine Content of Psilocybe Cubensis (Earle) Singer. Fungal Biol 2024, 128, 1590–1595, doi: 10.1016/J.FUNBIO.2023.12.001.
3. Hernandez-Leon, A. et al. Antidepressant- and Anxiolytic-Like Activities and Acute Toxicity Evaluation of the Psilocybe Cubensis Mushroom in Experimental Models in Mice. J Ethnopharmacol 2024, 320, 117415–117415, doi: 10.1016/j.jep.2023.117415.
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Copyright (c) 2025 Beatriz P. Senra, Johannes H. Bartholomaeus, Ricardo Jorge Dinis-Oliveira, Carlos J. A. Ribeiro
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