Small Scale Testing of Poultry Feces to Enumerate the Risk of Environmental Contamination & Food-Borne Illnesses
DOI:
https://doi.org/10.48797/sl.2026.399Keywords:
Poultry Feces; Environmental Contamination; Food-borne Illnesses; MicroorganismsAbstract
Live poultry stocks from local grocery markets are a potent reservoir of environmental contamination and foodborne illness. Bacteria present in poultry feces pose a potential health risk to humans through cross-contamination, which may lead to the spread of infectious diseases. The present study specifically focused on bacteria associated with poultry feces. We aimed to conduct microbiological analysis to isolate and identify microbial load present in poultry fecal samples that could lead to environmental contamination and associated risks of foodborne illnesses. Isolation from fecal samples was performed through standard culture techniques, and differential and biochemical analyses were performed as isolation and identification methods. Twenty fecal samples were collected, and the findings of this study were as follows: Staph spp. 100%, E. coli spp. 25%, Serratia spp. 10%, Salmonella spp. 15% and Citrobacter spp. 20%. The findings of this study provided insight into the microbial association with live poultry stocks in grocery markets and their potential wellness concerns.References
Nasrin, M.S.; Islam, M.J.; Nazir, K.H.M.N.H.; Choudhury, K.A.; Rahman, M.T. Identification of Bacteria and Determination of Their Load in Adult Layer and Its Environment. Journal of the Bangladesh Society for Agricultural Science and Technology 2007, 69–72.
Bano, R.; Shah, H.; Sharif, M.; Akhtar, W. An Overview of the Pakistan Poultry Sector; 2009;
Chang, H.-S. Analysis of the Philippine Chicken Industry: Commercial versus Backyard Sectors. Asian Journal of Agriculture and Development 2007, 4, 41–56, doi:10.37801/ajad2007.4.1.4.
Mahami, T.; Togby-Tetteh, W.; Kottoh, D.I.; Amoakoah-Twum, L.; Gasu, E.; Annan, S.N.Y.; Larbi, D.; Adjei, I.; Adu-Gyamfi, A. Microbial Food Safety Risk to Humans Associated with Poultry Feed: The Role of Irradiation. Int. J. Food Sci. 2019, 2019, 1–7, doi:10.1155/2019/6915736.
Kadariya, J.; Smith, T.C.; Thapaliya, D. Staphylococcus Aureus and Staphylococcal Food-Borne Disease: An Ongoing Challenge in Public Health. Biomed Res. Int. 2014, 2014, 827965, doi:10.1155/2014/827965.
University of Washington Environmental Health and Safety Zoonotic Disease Prevention When Working with Poultry;
Todar, K. Pathogenic E. Coli. In Textbook of Bacteriology; Department of Bacteriology, University of Wisconsin–Madison: Madison, WI, USA, 2008.
Food-Borne Diseases: E. Coli 0157:H7 Available online: https://www.infoplease.com/math-science/health/diseases/food-borne-diseases-e-coli-0157h7 (accessed on 16 January 2026).
Bélanger, L.; Garenaux, A.; Harel, J.; Boulianne, M.; Nadeau, E.; Dozois, C.M. Escherichia Coli from Animal Reservoirs as a Po-tential Source of Human Extraintestinal Pathogenic E. Coli. FEMS Immunol. Med. Microbiol. 2011, 62, 1–10, doi:10.1111/J.1574-695X.2011.00797.X.
Kabir, S.M.L. Avian Colibacillosis and Salmonellosis: A Closer Look at Epidemiology, Pathogenesis, Diagnosis, Control and Public Health Concerns. Int. J. Environ. Res. Public Health 2010, 7, 89–114, doi:10.3390/ijerph7010089.
Cabral, J.P.S. Water Microbiology. Bacterial Pathogens and Water. Int. J. Environ. Res. Public Health 2010, 7, 3657–3703, doi:10.3390/ijerph7103657.
Petkov, D.; Collins-Emerson, J.; French, N. Characterisation of Salmonella (MAF Technical Paper No. 2011/67); Ministry of Agriculture and Forestry, 2010;
Rouger, A.; Tresse, O.; Zagorec, M. Bacterial Contaminants of Poultry Meat: Sources, Species, and Dynamics. Microorganisms 2017, 5, 50, doi:10.3390/microorganisms5030050.
Sharma, S.; Kaur, S.; Naguib, M.; Bragg, A.; Schneider, A.; Kulkarni, R.R.; Nazmi, A.; Abdelaziz, K. Major Foodborne Bacterial Pathogens in Poultry: Implications for Human Health and the Poultry Industry and Probiotic Mitigation Strategies. Microor-ganisms 2025, 13, 2363, doi:10.3390/microorganisms13102363.
Shaji, S.; Selvaraj, R.K.; Shanmugasundaram, R. Salmonella Infection in Poultry: A Review on the Pathogen and Control Strate-gies. Microorganisms 2023, 11, 2814, doi:10.3390/microorganisms11112814.
Młynarczyk, A.; Młynarczyk, G.; Pupek, J.; Bilewska, A.; Kawecki, D.; Łuczak, M.; Gozdowska, J.; Durlik, M.; Pączek, L.; Chmura, A.; et al. Serratia Marcescens Isolated in 2005 From Clinical Specimens From Patients With Diminished Immunity. Transplant. Proc. 2007, 39, 2879–2882, doi:10.1016/j.transproceed.2007.08.046.
David, M.D.; Weller, T.M.A.; Lambert, P.; Fraise, A.P. An Outbreak of Serratia Marcescens on the Neonatal Unit: A Tale of Two Clones. Journal of Hospital Infection 2006, 63, 27–33, doi:10.1016/j.jhin.2005.11.006.
Mahata, M.E.; Lestari, I.; Dharma, A.; Ryanto H., I.; Armenia; Rizal, Y. Use of Serratia Marcescens for Feed Processing: Broiler Performance and Pathogenicity Assay. Int. J. Poult. Sci. 2012, 11, 424–426, doi:10.3923/ijps.2012.424.426.
Guentzel, M.N. Escherichia, Klebsiella, Enterobacter, Serratia, Citrobacter, and Proteus. In Medical Microbiology; Baron, S., Ed.; University of Texas Medical Branch at Galveston, 1996 ISBN 0963117211.
Oakley, B.B.; Morales, C.A.; Line, J.; Berrang, M.E.; Meinersmann, R.J.; Tillman, G.E.; Wise, M.G.; Siragusa, G.R.; Hiett, K.L.; Seal, B.S. The Poultry-Associated Microbiome: Network Analysis and Farm-to-Fork Characterizations. PLoS One 2013, 8, e57190, doi:10.1371/journal.pone.0057190.
Cain, D.; Hanks, H.; Weis, M.; Bottoms, C.; Lawson, J. Microbiology Laboratory Manual Biol 2421L Available online: https://pt.scribd.com/document/257221438/Microbiology-Lab-Manual-Revised-Spring-2013.
Newell, D.G.; Koopmans, M.; Verhoef, L.; Duizer, E.; Aidara-Kane, A.; Sprong, H.; Opsteegh, M.; Langelaar, M.; Threfall, J.; Scheutz, F.; et al. Food-Borne Diseases — The Challenges of 20 Years Ago Still Persist While New Ones Continue to Emerge. Int. J. Food Microbiol. 2010, 139, S3–S15, doi:10.1016/j.ijfoodmicro.2010.01.021.
European Food Safety Authority (EFSA) Assessment of the Public Health Significance of Meticillin Resistant Staphylococcus Aureus (MRSA) in Animals and Foods. EFSA Journal 2009, 7, 1–73, doi:10.2903/j.efsa.2009.993.
Mgbeahuruike, A.C.; Agina, O.A.; Anyanwu, M.U.; Emejuo, N.T.; Ekere, S.O.; Ugwu, P.C.; Uju, C.N.; Andong, F.A. Microbial Con-tamination of Poultry Feed and the Effects on Birds’ Performance. Animal Research International 2023, 20, 4834–4861, https://www.ajol.info/index.php/ari/article/view/247059.
Woodward, D.L.; Clark, C.G.; Caldeira, R.A.; Ahmed, R.; Rodgers, F.G. Verotoxigenic Escherichia Coli (Vtec): A Major Public Health Threat in Canada. Canadian Journal of Infectious Diseases and Medical Microbiology 2002, 13, 321–330, doi:10.1155/2002/383840.
Кolchyk, O. V.; Borovuk, I. V.; Buzun, A.I.; Іllarionova, Т. V.; Zazharska, N.M. Microorganisms’ Growth Inhibition in Poultry Meat Using Bacillus Spp. World’s Veterinary Journal 2024, 14, 424–434, doi:10.54203/scil.2024.wvj50.
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