Molecular characterization of thyroid tumors of dogs – a multicentric Portuguese series

Authors

  • A. Monteiro Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), 4200-135 Porto, Portugal; University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
  • T. B. Gaspar Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), 4200-135 Porto, Portugal; Departamento de Ciências Veterinárias (DCV), Escola Universitária Vasco da Gama (EUVG), 3020-210 Coimbra, Portugal
  • M. Pinto Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), 4200-135 Porto, Portugal
  • I. Pires University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal; CECAV-Veterinary and Animal Research Center, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal; Department of Veterinary Science of the University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
  • P. Soares Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), 4200-135 Porto, Portugal; Medical Faculty of the University of Porto (FMUP), 4200-319 Porto, Portugal
  • C. Tavares Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (Ipatimup), 4200-135 Porto, Portugal; TOXRUN – Unidade de Investigação em Toxicologia, Instituto Universitário de Ciências da Saúde, CESPU, CRL, 4585-116 Gandra, Portugal https://orcid.org/0000-0003-0140-2098

DOI:

https://doi.org/10.48797/sl.2023.67

Keywords:

Poster

Abstract

Background: The incidence of thyroid carcinoma (TC) in human population has been increasing worldwide, and it was estimated an incidence of 2.1% of cancer new cases in 2019 [1]. A smaller incidence is reported for canine population (1.1%) in 1995-2005 [2]. Diagnosis, prognosis, and management of human TC rely on mutation screening of BRAF, RAS genes, and TERT promoter. BRAF, NRAS, HRAS, and KRAS encode proteins that are key effectors of MAPK signaling pathway, an important kinase pathway, conserved in mammals [3]. Objective: Our goal was to explore the canine TC’s oncobiology, and to verify whether natural occurring canine TC could (or not) be set as suitable model to study its human homolog. Methods: We collected 57 samples (5 adenomas (9%), and 52 carcinomas (91%)), from which we performed DNA extraction from formalin-fixed paraffin-embedded tissues, PCR, and Sanger sequencing of exon 16 of BRAF (n = 49), exon 2 of NRAS (n = 41), exon 3 of HRAS (n = 41), and exon 3 (n = 31) and 4 (n = 20) of KRAS. Results: We detected silent mutations on HRAS (p.N47=) (n = 14/41, 34%) and NRAS (p.E63=) (n = 1/41, 2.4%), however, no mutations were found in the other genes. Conclusions: Our results corroborate those described by Campos et al. (2014) [4]. Nevertheless, both studies only evaluated the homologous regions of the hotspots of human most common TC mutations. We cannot exclude the hypothesis that in dogs, those genes can present activating mutations in other exons, different from human’s hotspots.

References

1. www.seer.cancer.gov

2. Katja L Wucherer, Vicki Wilke. Thyroid cancer in dogs: an update based on 638 cases. J Am Anim Hosp Assoc. 2010. 46, 249-54.

3. Cuenda, Ana. Mitogen-Activated Protein Kinases (MAPK) in Cancer. Reference Module in Biomedical Sciences (2018). 10.1016/B978-0-12-801238-3.64980-2.

4. M. Campos, M.M.J. Kool, S. Daminet, R. Ducatelle, G. Rutteman, H.S. Kooistra, S. Galac. Upregulation of the PI3K/Akt Pathway in the Tumorigenesis of Canine Thyroid Carcinoma. J.A. Mol. 2014, 28, 1814-1823.

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Published

2023-04-21

How to Cite

Monteiro, A., Gaspar, T. B., Pinto, M., Pires, I., Soares, P., & Tavares, C. (2023). Molecular characterization of thyroid tumors of dogs – a multicentric Portuguese series. Scientific Letters, 1(Sup 1). https://doi.org/10.48797/sl.2023.67

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