Metalloproteinases and cellular components in saliva from periodontitis patients: preliminary study

Authors

  • S. Aguilera IUCS, CESPU, 4585-116 Gandra, Portugal
  • M. Relvas UNIPRO - Oral Pathology and Rehabilitation Research Unit, IUCS, CESPU, 4585-116 Gandra, Portugal
  • A. Viana da Costa TOXRUN - Toxicology Research Unit, IUCS, CESPU, 4585-116 Gandra, Portugal

DOI:

https://doi.org/10.48797/sl.2023.80

Keywords:

Poster

Abstract

Background: Metalloproteinases (MMPs) are extracellular matrix macromolecules important in cellular development and morphogenesis, being capable of activating growth factors nearby, cell surface receptors, adhesion molecules and immune mediators [1]. Because type I collagen is the major component of the periodontal extracellular matrix, special attention is given to the role of collagenases, among them MMP-8, which is involved in the degradation of this matrix. Periodontitis (PD) is a destructive inflammatory disease of the supporting tissues of the teeth, affecting 11% of the world’s population. Disease results from the interaction between the oral biofilm and the immune system's response [2]. Moreover, salivary MMP levels are associated with aggravation of periodontitis [3, 4], but no relation with which leukocytes populations yet. Objective: Our aim is to evaluate MMP-8 salivary levels and the corresponding leukocyte populations in patients with periodontitis stage I/II, stage III/IV and healthy controls. Methods: The study includes patients from the Dental Clinic appointments of the University Clinic of IUCS (7 healthy, 5 stage I/II, 7 stage III/IV). Collection of unstimulated saliva samples, followed by quantification of MMP-8 by ELISA and cellular recovery for leucocyte analysis by flow cytometry. Results: In a small sample of 19 individuals, studied so far, we observed that PD patients had higher MPP-8 levels than healthy controls. For cellular analysis, only 3 samples were studied, 3 healthy individuals. However, it has not yet been possible to establish any association with MMP-8 levels. Conclusions: In a near future, we expect our results to allow a possible association between MMP-8 salivary levels and periodontitis disease expression - stage versus grade. Flow cytometry analyses will allow for the evaluation of the leukocyte populations in saliva and their link with MMP-8 levels, in order to find a potential biomarker for periodontitis.

References

1. Visse, R.; Nagase, H. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ Res. 2003, 92, 827-839.

2. Hajishengallis, G.; Korostoff, J.M. Revisiting the Page & Schroeder model: The good, the bad and the unknowns in the periodontal host response 40 years later. Periodontology 2000 2017, 75, 116-151.

3. Ebersole, J.L.; Schuster, J.L.; Stevens, J.; et al. Patterns of salivary analytes provide diagnostic capacity for dis-tinguishing chronic adult periodontitis from health. J Clin Immunol 2013, 33, 271-279.

4. Zhang L; Xiue L; Yan H; Huang L. Salivary matrix metalloproteinase (MMP)-8 as a biomarker for periodontitis: A PRISMA-compliant systematic review and meta-analysis. Medicine (Baltimore). 2018, 97(3): e9642.

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Published

2023-04-21

How to Cite

Aguilera , S., Relvas , M., & Viana da Costa , A. (2023). Metalloproteinases and cellular components in saliva from periodontitis patients: preliminary study . Scientific Letters, 1(Sup 1). https://doi.org/10.48797/sl.2023.80

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