In vitro and in silico evaluation of psilocybin and psilocin’s interaction with CYP450 enzymes

Authors

  • Andreia Machado Brito-da-Costa Associate Laboratory i4HB - Institute for Health and Bioeconomy, Portugal; UCIBIO - Applied Molecular Biosciences Unit, Translational Toxicology Research Laboratory, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal; UCIBIO – Applied Molecular Biosciences Unit, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal https://orcid.org/0000-0001-7327-1598
  • Mariana Carvalho Associate Laboratory i4HB - Institute for Health and Bioeconomy, Portugal; UCIBIO - Applied Molecular Biosciences Unit, Translational Toxicology Research Laboratory, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal
  • Ricardo Jorge Dinis-Oliveira Associate Laboratory i4HB - Institute for Health and Bioeconomy, Portugal; UCIBIO - Applied Molecular Biosciences Unit, Translational Toxicology Research Laboratory, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal; FOREN-Forensic Science Experts, Dr. Mário Moutinho Avenue, n.° 33-A, Lisbon, Portugal; Department of Public Health and Forensic Sciences, and Medical Education, Faculty of Medicine, University of Porto, Porto, Portugal
  • Áurea Madureira-Carvalho Associate Laboratory i4HB - Institute for Health and Bioeconomy, Portugal; UCIBIO - Applied Molecular Biosciences Unit, Forensics and Biomedical Sciences Research Laboratory, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal
  • Sérgio F. Sousa LAQV/REQUIMTE, BioSIM – Biomedicine Department, Faculty of Medicine, University of Porto, Porto, Portugal https://orcid.org/0000-0002-6560-5284
  • Diana Dias da Silva Associate Laboratory i4HB - Institute for Health and Bioeconomy, Portugal; UCIBIO – Applied Molecular Biosciences Unit, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal; UCIBIO - Applied Molecular Biosciences Unit, Forensics and Biomedical Sciences Research Laboratory, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal; LAQV/REQUIMTE, ESS, Polytechnic of Porto, Rua Dr. António Bernardino de Almeida, 400 4200-072, Porto https://orcid.org/0000-0002-7331-9157

DOI:

https://doi.org/10.48797/sl.2024.236

Keywords:

Poster

Abstract

Background: Psilocybin is a hallucinogen produced by “magic mushrooms”, being rapidly metabolized in the organism into psilocin [1, 2]. A scientific gap exists regarding the possible interactions between psilocybin/psilocin and CYP450 enzymes. Given their biological importance, and since the binding of drugs to CYP450 enzymes can interfere with the metabolism of other substrates leading to drug-drug interactions, this research topic is of utmost importance. Objective: This study aimed to evaluate in silico and in vitro the interaction of psilocybin and psilocin with the enzymes CYP2A6/2B6/2D6/2E1/3A4. Methods: The in vitro assessment of inhibition was performed using the Vivid®CYP450 screening kits. IC50 was calculated using GraphPad Prism 9.3.0. For in silico assessment, molecular dynamics were performed using the PMEMD.cuda module in AMBER16. Calculations were made on the last 100 ns of the trajectory (stable zone) to assess the interaction between enzyme and ligand, namely MMGBSA, per-residue decomposition energy, and hydrogen bonds. Results: Psilocin showed the capacity to be an inhibitor of CYP2A6/2B6/2D6/2E1/3A4, based on the respective IC50 values (mM) of 2.06, 6.17, 11.89, 6.37, and 2.36. Considering the MMGBSA, higher values were obtained for psilocin, corroborating the stronger binding affinity of this compound. The interaction of psilocybin/psilocin with CYP2A6 is mediated by a hydrogen bond established with the protein residue Asn297. Other important residues include Phe107 and Ile366. For CYP2B6, the strong binding of psilocin is mediated by interactions with Ile114, Thr302 (hydrogen bond), and Leu363. For interaction with CYP2D6, the most important residue seems to be Ser304, with which it forms a hydrogen bond; for CYP2E1, key residues include Phe207, Thr303, and Phe478. A strong hydrogen bond is formed between psilocin and CYP3A4 residue Phe304, contributing to the high binding affinity. Conclusions: The results suggest a potential for psilocin to inhibit all enzymes, especially CYP2A6 and CYP3A4.

References

1. Brito-da-Costa, A.M.; Dias da Silva, D.; Madureira-Carvalho, Á.; Dinis-Oliveira, R.J. Psilocybin and magic mushrooms: Patterns of abuse and consequences of recreational misuse. In Handbook of Substance Misuse and Addictions; Patel, V.B.; Preedy, V.R., Eds.; Springer Nature: Switzerland, (2022).

2. Dinis-Oliveira, R.J. Metabolism of psilocybin and psilocin: clinical and forensic toxicological relevance. Drug Metab Rev (2017), 49, 84-91.

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Published

2024-05-01

How to Cite

Machado Brito-da-Costa, A., Carvalho, M., Dinis-Oliveira, R. J., Madureira-Carvalho, Áurea, F. Sousa, S., & Dias da Silva, D. (2024). In vitro and in silico evaluation of psilocybin and psilocin’s interaction with CYP450 enzymes. Scientific Letters, 1(Sup 1). https://doi.org/10.48797/sl.2024.236

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