Diagnostic methods of leishmaniosis in dogs: state-of-the-art

Authors

  • C. P. Penelas Costa Animal and Veterinary Research Centre (CECAV), Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal https://orcid.org/0000-0002-2994-4940
  • J. C. Prata TOXRUN—Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL, 4585-116 Gandra, Portugal; Food Microbiology and Technology Laboratory, Department of Aquatic Production, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal
  • L. Cardoso Animal and Veterinary Research Centre (CECAV), Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 5000-801 Vila Real, Portugal
  • T. G. Barroso TOXRUN—Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL, 4585-116 Gandra, Portugal; INESC TEC—Institute for Systems and Computer Engineering, Technology and Science, Campus da FEUP, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal

DOI:

https://doi.org/10.48797/sl.2023.68

Keywords:

Poster

Abstract

Background: Canine leishmaniosis (CanL) is caused by Leishmania infantum transmitted by the bite of phlebotomine sand flies [1]. Objective: This systematic review aims at providing the most updated information about the laboratory diagnosis of leishmaniosis in dogs using traditional methods and innovative techniques. Methods: A bibliographic search was performed on 23 February 2023 in Scopus targeting all currently available literature up to 22 February 2023 using the following keywords: ("Canine leishmaniosis" OR "Leishmania" OR "Leishmaniosis in Dogs" OR "Visceral leishmaniasis" OR "cutaneous leishmaniasis" OR "leishmaniasis") AND ("traditional diagnosis" OR "biochemical profile" OR "clinical analysis" OR "complete blood count (CBC)" OR "cytology" OR "hemogram" OR "PCR" OR "serology" OR "urinalysis") AND ("innovative diagnostic techniques" OR "molecular diagnostic test" OR "rapid diagnostic tests" OR "spectroscopy POC") AND ("diagnostic performance"). Results: CanL laboratory diagnosis is traditionally carried out directly or indirectly, with methods including molecular techniques, quantitative serological methods, specific serology and also the evaluation of hematological and biochemical parameters [2]. Innovative techniques address point-of-care applications, e.g.DNA-probes based on gold nanoparticles. Conclusions: Traditional diagnostic methods vary in their simplicity of use,however, these methods require large sample volumes, which cannot always be collected from patients and may pose welfare concerns. Innovative techniques should provide high method accuracy while using low sample volumes and being reagentless, reducing costs and applying green chemistry principles.

References

1. Torrent, E.; Planellas, M.; Ordeix, L.; Pastor, J.; Rodon, J.; Solano-Gallego L. Serum Symmetric Dimethylarginine as an Early Marker of Excretory Dysfunction in Canine Leishmaniosis (L. infantum) Induced Nephropathy. Vet Med Int 2018, 7517359.

2. Salant, H.; NachumBiala, Y.; Feinmesser, B.; Perelmutter, M.; Baneth, G. Early onset of clinical leishmaniosis in a litter of pups with evidence of in utero transmission. Parasit. Vectors 2021,14, 326.

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Published

2023-04-21

How to Cite

Penelas Costa, C. P., Prata, J. C., Cardoso, L., & Barroso, T. G. (2023). Diagnostic methods of leishmaniosis in dogs: state-of-the-art. Scientific Letters, 1(Sup 1). https://doi.org/10.48797/sl.2023.68

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