Butylone enantioseparation and ecotoxicity evaluation on Daphnia magna

Authors

  • A. R. Carvalho TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, IUCS-CESPU, CRL, 4585-116 Gandra, Portugal; CISA, ESS, Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 400, 4200-072 Porto, Portugal https://orcid.org/0000-0003-0634-603X
  • A. M. Morão TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, IUCS-CESPU, CRL, 4585-116 Gandra, Portugal
  • V. M. F. Gonçalves TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, IUCS-CESPU, CRL, 4585-116 Gandra, Portugal; UNIPRO-Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences (IUCS), CESPU, 4585-116 Gandra, Portugal
  • M. E. Tiritan TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, IUCS-CESPU, CRL, 4585-116 Gandra, Portugal; Interdisciplinary Center of Marine and Environmental Research (CIIMAR), University of Porto,4450-208 Matosinhos, Portugal; Laboratory of Organic and Pharmaceutical Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal
  • A. R. Lado LSRE-LCM - Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials, Faculty of Engi-neering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal
  • J. S. Carrola Department of Biology and Environment, University of Trás-os-Montes and Alto Douro, CITAB, Vila Real, Portugal; Inov4Agro – Institute for Innovation, Capacity Building and Sustainability of Agri-food Production
  • M. M. Amorim CISA, ESS, Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 400, 4200-072 Porto, Portugal
  • C. Ribeiro TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, IUCS-CESPU, CRL, 4585-116 Gandra, Portugal https://orcid.org/0000-0001-7245-4552

DOI:

https://doi.org/10.48797/sl.2023.13

Keywords:

Poster

Abstract

Background: The synthetic drug butylone (2-methylamino-1-(3,4-methylenedioxyphenyl)butan-1-one, BTL) is a chiral cathinone consumed in the form of racemate [1]. After consumption, BTL is metabolized and excreted along with its metabolites, and its residues are carried by sewerage systems to wastewater treatment plants (WWTPs) [2]. Both human metabolism and biodegradation in WWTPs may be enantioselective causing a change in its enantiomeric fraction (EF). However, enantiomers may exhibit different biological activities including toxicity on non-target aquatic organisms [3]. Objective: This study aimed to separate both enantiomers and assess the sub-chronic effects on Daphnia magna focusing on morphophysiological and reproductive parameters. Methods: The enantiomers were separated by liquid chromatography using a homemade semipreparative chiral column (APS-Nucleosil coated with a 3,5-dimethylphenylcarbamate of amylose). Daphnia (with less than 24 h) were exposed for 9 days to concentrations of 0.1, 1, 10 μg/L, with a total of 5 replicates per concentration and a control.  Results: Morphophysiological alterations were observed, except in the heart area. A tendency to the increase of body size, heart size and mortality were observed for the higher concentrations (1 and 10 μ/L). The daphniids with eggs tended to decrease. Analysis of other endpoints (ongoing) are required to draw accurate conclusions. Conclusions: The present study demonstrates that exposure to BTL may cause effects on mortality and morphology of D. magna. The ongoing studies will bring new knowledge on BTL adverse effects and the possible enantioselective toxicity effects on this non-target aquatic organism.

References

1. Roque Bravo, R.; Carmo, H.; Valente, M.J.; Silva, J.P.; Carvalho, F.; Bastos, M.L.; Dias da Silva, D. From street to lab: in vitro hepatotoxicity of buphedrone, butylone and 3,4-DMMC. Arch Toxicol 2021, 95, 1443-1462.

2. Wang, Z.; Tang, B.; Wang, K.; Hao, Y.; Yang, F. Accumulation and risk prioritization of psychoactive substances in the critically endangered Yangtze finless porpoise. J. Hazard. Mater. 2023, 442, 130002.

3. Langa, I.; Tiritan, M.E.; Silva, D.; Ribeiro, C. Gas Chromatography Multiresidue Method for Enantiomeric Fraction Determination of Psychoactive Substances in Effluents and River Surface Waters. Chemosensors 2021, 9, 224.

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Published

2023-04-21

How to Cite

Carvalho, A. R., Morão, A. M., Gonçalves, V. M. F., Tiritan, M. E., Lado, A. R., Carrola, J. S., Amorim, M. M., & Ribeiro, C. (2023). Butylone enantioseparation and ecotoxicity evaluation on Daphnia magna. Scientific Letters, 1(Sup 1). https://doi.org/10.48797/sl.2023.13

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