Changes on swimming behavior induced by 3,4-methylenedioxypyrovalerone (MDPV) enantiomers on Daphnia magna
DOI:
https://doi.org/10.48797/sl.2023.36Keywords:
PosterAbstract
Background: Synthetic cathinones (SC) are a group of novel illicit psychotropic substances that have been found in the aquatic environment at low concentrations [1] and nonetheless they can impact aquatic invertebrates [2]. SC can pose unwanted diverse adverse effects such as behavioral toxicity to non-target organisms [3]. The 3,4-methylenedioxypyrovalerone (MDPV) is a chiral SC with psychotropic properties similar to methamphetamine and is traded as “bath salts” [4]. MDPV may be found in distinct forms, racemate or its enantiomers, that may exhibit different biological activities [2, 5]. Nevertheless, MDPV enantiotoxicity continued to be ignored as well as its impact on freshwater aquatic organisms, including invertebrates. Objective: This work aimed to assess the potential adverse effects of (R)-MDPV and (S)-MDPV on the swimming behavior of Daphnia magna. Methods: For that, neonates (< 24 hours) were exposed to 0.1 and 1.0 µg L-1 of both MDPV enantiomers for 5 days (5 replicates with 20 organisms each). After the end of exposure, 6 organisms of each replicate were randomly collected, placed into a 6-well plate (with ≈ 5 mL of the respective exposure medium) and video recorded for 1 minute. Parameters such as swimming speed, total distance travelled, and active time were evaluated using TheRealFishTracker program. Results: A significant increase in swimming speed was observed for the organisms exposed only to (S)-MDPV. On the contrary, an increase in active time was found in the organisms exposed to (R)-MDPV. No changes were detected for the total distance travelled for both enantiomers. Conclusions: This study showed that MDPV can interfere with the swimming behavior pattern of daphnia and that effects are enantioselective. However, for a better understanding of the enantioselective toxicity of MDPV on the fitness of daphnia, other parameters should be included (i.e., morphophysiological, reproductive and biochemical).
References
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Copyright (c) 2023 A. Pérez-Pereira, A. Carvalho, B. Castro, J. S. Carrola, C. Fernandes, M. Tiritan, C. Ribeiro
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