High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge

Authors

  • S. Quinteira TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL., Avenida Central de Gandra 1317, 4585-116 Gandra PRD, Portugal; BIOPOLIS/CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Rua Padre Armando Quintas, nº 7, 4485-661, Vairão, Portugal; Departamento de Bi-ologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal
  • A. B. Dias University Institute of Health Sciences, CESPU, Avenida Central de Gandra 1317, 4585-116 Gandra PRD, Portugal
  • L. Pinho Department of Veterinary Clinics, Abel Salazar Biomedical Sciences Institute, University of Porto, Porto, Portugal
  • N. V. Brito TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL., Avenida Central de Gandra 1317, 4585-116 Gandra PRD, Portugal
  • C. Campos Instituto Português de Oncologia do Porto Francisco Gentil, 4200-072, Porto, Portugal; Escola Superior de Saúde, Instituto Politécnico do Porto, 4200-072, Porto, Portugal
  • A. R. Freitas TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL., Avenida Central de Gandra 1317, 4585-116 Gandra PRD, Portugal; UCIBIO/REQUIMTE, Applied Molecular Biosciences Unit, Department of Bi-ological Sciences, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto, Porto, Portugal; Associate Laboratory i4HB, Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, Portugal
  • C. Miranda TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL., Avenida Central de Gandra 1317, 4585-116 Gandra PRD, Portugal, Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisbon, Caparica, Portugal

DOI:

https://doi.org/10.48797/sl.2023.77

Keywords:

Poster

Abstract

Background: Escherichia coli is currently a leading pathogen for deaths associated with antimicrobial resistance (AMR) [1]. AMR related to food-producing animals is a public health risk requiring a One Health approach [1,2]. Animals are major reservoirs of antibiotic resistant E. coli which can easily reach humans through the food chain, direct contact, or the environment [3]. Objective: To analyze the AMR profiles of E. coli in fecal samples from Holstein-Friesian cattle, as information on AMR from dairy farming in Portugal is scarce. Methods: Samples (n=112) collected from 7 different farms at Northern Portugal were pooled during February-March 2023 based on age group (8 calves and 8 cows per farm). Characteristic E. coli colonies were selected from MacConkey Agar supplemented with or without antibiotics (4 µg/ml cefotaxime; 3 µg/ml colistin), for confirmation by MALDI-TOF mass spectrometry and antimicrobial susceptibility testing (AST), according to EUCAST/CLSI guidelines. ESBL phenotype were searched using the Double-Disk Synergy Test. Results: Presumptive E. coli isolates (n=110) representing different farming production systems were obtained and 51 representatives were identified by MALDI-TOF and tested by AST. From both calves and cows, 95% and 63% of the E. coli isolates exhibited resistance to ≥1 antimicrobial classes and multidrug resistance (MDR, resistance to ≥3 antimicrobial classes), respectively. Isolates were mostly resistant to gentamycin (88%), ampicillin (72%), amoxicillin plus clavulanic acid (63%), tetracycline (63%) and cefotaxime (53%). Only two isolates showed susceptibility two all tested antibiotics. ESBL activity was observed in 21% of E. coli isolates. All analyzed pools were classified as MDR. Conclusions: This is one of the first studies on AMR rates of E. coli from dairy cattle in Northern Portugal.  It reveals a high prevalence of MDR E. coli, which is a worrying finding and emphasize the need of a multisectoral One Health approach to minimize its impact.

References

1. Aslam B, Khurshid M, Arshad MI, Muzammil S, Rasool M, Yasmeen N, Shah T, Chaudhry TH, Rasool MH, Shahid A, Xueshan X and Baloch Z. Antibiotic Resistance: One Health one world outlook. Front Cell Infect Microbiol 2021,11,771510.

2. Ma F, Xu S, Tang Z, Li Z, Zhang L. Use of antimicrobials in food animals and impact of transmission of antimi-crobial resistance on humans. Biosafety and Health 2021,3,32-38.

3. Samtiya M, Matthews KR, Dhewa T, Puniya AK. Antimicrobial resistance in the food chain: trends, mechanisms, pathways, and possible regulation strategies. Foods 2022,11,2966.

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Published

2023-04-21

How to Cite

Quinteira , S., Dias , A. B., Pinho , L., Brito , N. V., Campos , C., Freitas , A. R., & Miranda , C. (2023). High occurrence of multidrug-resistant Escherichia coli from Holstein-Friesian cattle in Northern Portugal: a One Health challenge . Scientific Letters, 1(Sup 1). https://doi.org/10.48797/sl.2023.77

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